Intermediate Layer Contribution in Placental Tissue

Annelise Roy & Sarah Griffiths, PhD

Placental membrane (PM) allografts are commonly used to treat chronic wounds. Native PM is composed of an amnion, chorion, and intermediate layer (IL) that contain matrix structures and regulatory components beneficial in wound healing. Historically, commercially available allografts were composed of only one or two layers of the PM. To maximize the conserved material in PM allografts, a dehydrated complete human placental membrane (dCHPM) allograft processed using the Clearify™ process was developed. Histological and proteomic characterization comparing dCHPM allografts with native PM demonstrated that the majority of matrix structures and regulatory proteins are retained in dCHPM allografts...

The Use of a Dehydrated Complete Human Placental Membrane Allograft for Mohs Surgical Defects of the Nose

Kelly M Wilmas, MD, Jigar Patel, MD, Sirunya Silapunt, MD, Hung Q Doan, MD, PhD, Michael R Migden, MD

Background: Repair options for Mohs surgical defects include primary closure, flap or graft, or healing by second intention. These options may not be optimal in all cases. A dehydrated complete human placental membrane (dCHPM) allograft may serve as an alternative repair option. Objective: To assess the aesthetic and functional outcomes of an alternative repair technique for Mohs surgical defects of the nose. Methods: Twenty patients with Mohs surgical defects of the nose repaired with a dCHPM allograft were retrospectively identified. Photographs were used to demonstrate surgical technique and outcomes. Two blinded observers evaluated final outcomes using the Patient...

Placental Tissues as Biomaterials in Regenerative Medicine

Annelise RoyMorgan MantayCourtney BrannanSarah Griffiths, PhD

Placental tissues encompass all the tissues which support fetal development, including the placenta, placental membrane, umbilical cord, and amniotic fluid. Since the 1990s there has been renewed interest in the use of these tissues as a raw material for regenerative medicine applications. Placental tissues have been extensively studied for their potential contribution to tissue repair applications. Studies have attributed their efficacy in augmenting the healing process to the extracellular matrix scaffolds rich in collagens, glycosaminoglycans, and proteoglycans, as well as the presence of cytokines within the tissues that have been shown to stimulate re-epithelialization...